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KMID : 1140120110160030255
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Cancer Prevention Research
2011 Volume.16 No. 3 p.255 ~ p.262
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Widdrol Induces Apoptosis in Human Cervical Carcinoma HeLa Cells
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Yun Hee-Jung
Jung Jong-Hun
Kwon Hyun-Ju
Hyun Sook-Kyung
Choi Yung-Hyun
Park Jung-Ha
Kim Byung-Woo
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Abstract
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In this study, the anticancer activity of widdrol, essential oil compound isolated from Juniperus chinensis, is investigated in human cervical cancer HeLa cells. Widdrol inhibits the growth of HeLa cells in a dose- and time-dependent manner. To confirm whether these effects of widdrol are associated with apoptosis, apoptotic features and expression of apoptosis-related proteins are examined. As a result, typical morphological characteristics of apoptotic cells such as formation of apoptotic body was observed as measured by DAPI staining. In Western blot analysis, the expression of anti-apoptotic Bcl-2 and Bcl-XL were decreased and cytosolic cytochrome c was increased in cells treated with widdrol. Also, caspase-8 and caspase-9, which are initiator caspase each in extrinsic and intrinsic pathway, were activated, activation of executioner caspase (caspase-3) and proteolytic cleavage of PARP were also observed. Therefore, widdrol seems to induce apoptosis by activating both intrinsic and extrinsic pathway in HeLa cells at the same time.
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KEYWORD
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Widdrol, HeLa cells, Apoptosis, Intrinsic and extrinsic apoptotic pathway, Caspase cascade
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